Wang E, Cairncross J G, Liem R K
Proc Natl Acad Sci U S A. 1984 Apr;81(7):2102-6. doi: 10.1073/pnas.81.7.2102.
We have used a human glioma cell line (U-251MG) to study the expression and cytoplasmic organization of vimentin (decamin) and the glial filament protein (GFP). Four clones of the parental U-251 cultures were isolated and found to express GFP from 1-2% to 99% of the cells in the population. Double immunofluorescence microscopy with antibodies to vimentin and GFP has shown that, in all four clonal cell lines, vimentin-containing filaments are expressed in most cells as an organized network and, in GFP-positive cells, GFP and vimentin are associated with the same filament network. Immunoelectron microscopy with specific antibodies labeled with colloidal gold particles of various sizes shows that GFP and vimentin are localized in the same filaments. These findings confirm in vitro studies of the copolymerization of subunits of different biochemical nature into the same intermediate filament and suggest the in vivo probability of the coassembly of GFP and vimentin from a possible soluble pool of monomers.
我们使用了一种人类胶质瘤细胞系（U - 251MG）来研究波形蛋白（结蛋白）和神经胶质纤维酸性蛋白（GFP）的表达及细胞质组织。从亲代U - 251培养物中分离出四个克隆，发现群体中1% - 2%至99%的细胞表达GFP。用针对波形蛋白和GFP的抗体进行双重免疫荧光显微镜检查表明，在所有四个克隆细胞系中，含波形蛋白的细丝在大多数细胞中以有组织的网络形式表达，并且在GFP阳性细胞中，GFP和波形蛋白与同一细丝网络相关联。用标记有各种大小胶体金颗粒的特异性抗体进行免疫电子显微镜检查表明，GFP和波形蛋白定位于同一细丝中。这些发现证实了关于不同生化性质的亚基共聚形成同一中间丝的体外研究，并提示了从可能的可溶性单体库中GFP和波形蛋白共同组装的体内可能性。
