Wolf N S, MacMillan J R, Priestley G V
Blood Cells. 1983;9(3):515-33.
A series of experiments were conducted to approach the question of whether CFU-S decline in self-replicative potential and in life saving potential in the serial transplant recipient were the result of events intrinsic to the CFU-S lineage or were the direct result of incidental external events. Specifically, the possibilities of CFU-S death or loss of potential related to in vitro manipulation, related to failure of the most capable CFU-S to lodge in the bone marrow, related to failure of accessory cells to lodge or related to and coincident with increased CFU-S cycling were all examined. Only the last named factor was associated with CFU-S decline. The results were compatible with the generation age concept i.e., that the loss of CFU-S self replicative potential was due to a permanent shift of the most "primitive" CFU-S to a more differentiated and less self-replicative status when an extreme and prolonged demand for replenishment of the more differentiated CFU-S pools exists.
进行了一系列实验，以探讨在连续移植受者中，集落形成单位脾（CFU-S）的自我复制潜力和挽救生命潜力的下降是CFU-S谱系内在事件的结果，还是偶然外部事件的直接结果。具体而言，研究了与体外操作相关的CFU-S死亡或潜力丧失的可能性，与最有能力的CFU-S无法在骨髓中着床相关的可能性，与辅助细胞无法着床相关的可能性，以及与CFU-S循环增加相关并同时发生的可能性。只有最后提到的因素与CFU-S下降有关。结果与生成年龄概念相符，即当对更分化的CFU-S池的补充存在极端且长期需求时，CFU-S自我复制潜力的丧失是由于最“原始”的CFU-S永久性地转变为更分化且自我复制能力更低的状态。
