Melzer P
Brain Res Bull. 1984 Mar;12(3):331-4. doi: 10.1016/0361-9230(84)90061-3.
Stimulus-specific neuronal responses in the central auditory pathway of Psammomys obesus were studied with deoxyglucose autoradiography. Responses were revealed mainly in the inferior colliculus. With whole head sectioning and subsequent gentle freeze-drying, excellent structural preservation was achieved. With the use of double-tritiated deoxyglucose, Ultrofilm 3H and vacuum-contact exposure, a mean spatial resolution of 43 micron Full Width Half Maximum was achieved.
利用脱氧葡萄糖放射自显影术研究了肥胖沙鼠中枢听觉通路中刺激特异性神经元反应。反应主要在下丘中显示出来。通过全脑切片及随后的温和冷冻干燥，实现了良好的结构保存。使用双标记氚脱氧葡萄糖、超薄膜3H和真空接触曝光，获得了43微米半高宽的平均空间分辨率。
