Lo T N, Almeida A P, Beaven M A
Eur J Pharmacol. 1984 Mar 16;99(1):31-43. doi: 10.1016/0014-2999(84)90429-1.
Chemotactic activity of protein origin was demonstrated in carrageenan-induced pleural exudates by the chemotactic response of neutrophils in the modified Boyden chamber. The activity was partly neutralized by monospecific antisera to complement component 5 and was destroyed by trypsin and chymotrypsin treatment but it differed from that in rat serum or plasma in that it was stable for 30 min at 56 degrees C. Indomethacin (5 mg/kg i.v.) reduced equally protein content (56%) and total chemotactic activity (58%); i.e., chemotactic activity/mg of exudate protein was unchanged. Intrapleural injection of autologous or homologous serum also induced an infiltration of neutrophils; the protein content of the pleural fluid decreased by 60-70% in 4 h, whereas with carrageenan there was a progressive increase in exudate protein. When serum was injected in two doses to maintain protein levels comparable to those found following carrageenan injection, the number of neutrophils in the exudates was also comparable. In contrast to carrageenan, the response to serum was not inhibited by indomethacin. From these and other data we suggest that the exudate chemotactic activity is generated from plasma protein and that indomethacin acts primarily to reduce extravasation of plasma and consequently generation of chemotactic activity.
通过改良的博伊登小室中嗜中性粒细胞的趋化反应，在角叉菜胶诱导的胸腔渗出液中证实了蛋白质来源的趋化活性。该活性部分被针对补体成分5的单特异性抗血清中和，并被胰蛋白酶和糜蛋白酶处理破坏，但它与大鼠血清或血浆中的趋化活性不同，因为它在56℃下30分钟内稳定。消炎痛（5mg/kg静脉注射）同样降低了蛋白质含量（56%）和总趋化活性（58%）；即，每毫克渗出液蛋白质的趋化活性未改变。胸腔内注射自体或同源血清也诱导了嗜中性粒细胞浸润；胸腔积液的蛋白质含量在4小时内降低了60-70%，而角叉菜胶处理后渗出液蛋白质呈逐渐增加。当分两剂注射血清以维持与角叉菜胶注射后相当的蛋白质水平时，渗出液中嗜中性粒细胞的数量也相当。与角叉菜胶不同，消炎痛不抑制对血清的反应。根据这些及其他数据，我们认为渗出液趋化活性由血浆蛋白产生，消炎痛主要作用是减少血浆外渗，从而减少趋化活性的产生。
