Slovick F T, Abboud C N, Brennan J K, Lichtman M A
Exp Hematol. 1984 Jun;12(5):327-38.
We have studied the survival of granulocytic and monocytic progenitor cells (CFU-GM) in human liquid marrow cultures. CFU-GM were present in the nonadherent cell population for a mean of 12 weeks without recharging . Histochemical analysis of agar gels revealed that most day-7 colonies were of neutrophilic type (CFU-N), whereas the majority of day-14 colonies were of mixed neutrophilic-macrophagic type (CFU-NM) for the first four weeks of culture and became predominantly of macrophagic type (CFU-M) thereafter. Eosinophilic colonies (CFU-Eo) declined after week 2 of culture. We have documented that CFU-GM were present in the adherent layer of these cultures, and that the CFU-GM in the nonadherent compartment arise from the adherent layer. In addition, we have compared the pre-CFU-GM survival in the adherent and nonadherent populations and determined that these progenitors were rapidly depleted from both compartments though their survival at the end of week 1 was better in the adherent than in the nonadherent layer.
我们已经研究了人液体骨髓培养物中粒细胞和单核细胞祖细胞（CFU-GM）的存活情况。CFU-GM存在于非贴壁细胞群体中，平均可持续12周而无需再接种。琼脂凝胶的组织化学分析显示，培养第7天的大多数集落为嗜中性类型（CFU-N），而培养第14天的大多数集落在培养的前四周为嗜中性-巨噬细胞混合类型（CFU-NM），此后主要变为巨噬细胞类型（CFU-M）。嗜酸性集落（CFU-Eo）在培养第2周后减少。我们已经证明CFU-GM存在于这些培养物的贴壁层中，并且非贴壁隔室中的CFU-GM来源于贴壁层。此外，我们比较了贴壁和非贴壁群体中前CFU-GM的存活情况，并确定这些祖细胞在两个隔室中均迅速耗竭，尽管它们在第1周末在贴壁层中的存活情况比在非贴壁层中更好。
