Kerényi T, Voss B, Rauterberg J, Fromme H G, Jellinek H
Exp Mol Pathol. 1984 Jun;40(3):380-90. doi: 10.1016/0014-4800(84)90055-8.
The microdistribution of type V collagen, fibronectin, and laminin on the luminal surface of perfusion-fixed normal rat aortic endothelium has been studied by an immunoelectron microscopic method using monospecific antibodies and a protein A-gold complex. Gold particles indicating the presence of these biologically active connective tissue proteins were localized in groups on and in the vicinity of the interendothelial border. They were also found on the small flaps of cell junctions as well as on certain cell projections and scattered on the cell surface. Correlative transmission electron-microscopic examinations proved the specificity of these localizations. The endothelial cells of the aorta differed markedly in the amount of scattered connective tissue proteins on their surface, suggesting that there are several types of aortic endothelial cells with distinct functional differences. The findings provide evidence that connective tissue proteins may contribute to the surface pattern of the normal endothelium, especially on cell borders. It is likely that these proteins influence functions such as the permeability and chemotactic activity of the endothelium pertinent to the development of vascular disease.
采用单特异性抗体和蛋白A-金复合物的免疫电子显微镜方法，研究了灌注固定的正常大鼠主动脉内皮腔表面V型胶原、纤连蛋白和层粘连蛋白的微分布。指示这些生物活性结缔组织蛋白存在的金颗粒成组地定位在内皮细胞间边界上及其附近。它们也出现在细胞连接的小皮瓣上以及某些细胞突起上，并散布在细胞表面。相关的透射电子显微镜检查证实了这些定位的特异性。主动脉内皮细胞表面散在的结缔组织蛋白数量有明显差异，这表明存在几种具有明显功能差异的主动脉内皮细胞类型。这些发现提供了证据，表明结缔组织蛋白可能有助于正常内皮的表面模式，尤其是在细胞边界处。这些蛋白很可能影响与血管疾病发展相关的内皮功能，如通透性和趋化活性。
