Takatori T, Tomii S, Terazawa K
J Forensic Sci. 1984 Apr;29(2):618-23.
Studies have been conducted on an enzymic fluorometric method based on an initial rate of reaction for the determination of choline. The reaction system consists of choline oxidase coupled to peroxidase and homovanillic acid. Concentrations of choline as low as 0.1 nmol could be detected by this procedure. The concentration of free choline in normal semen was 18.7 to 29.5 mumol/mL. Free choline in other body fluids was negligible. The choline concentrations in seminal stains maintained at room temperature were not changed during a 30-day period. Those concentrations in seminal fluids kept at room temperature were detected until at least the fifth day.
已经对基于反应初始速率测定胆碱的酶荧光法进行了研究。反应体系由与过氧化物酶和高香草酸偶联的胆碱氧化酶组成。通过该方法可以检测低至0.1 nmol的胆碱浓度。正常精液中游离胆碱的浓度为18.7至29.5 μmol/mL。其他体液中的游离胆碱可以忽略不计。室温下保存的精液污渍中的胆碱浓度在30天内没有变化。室温下保存的精液中的那些浓度至少在第五天仍可检测到。
