Räisänen M J
Life Sci. 1984 May 21;34(21):2041-5. doi: 10.1016/0024-3205(84)90368-0.
The N,N-dimethylated derivative of serotonin, bufotenin , is excreted into normal human urine as a free amine. Conjugation of bufotenin is, however, possible because of the phenolic hydroxyl group on the molecule. In the present study the urinary excretion of free and conjugated bufotenin of ten healthy, drug free subjects was examined. Acid as well as enzymatic hydrolysis was used to liberate the amine from its conjugate. Quantification was achieved by isotope dilution mass fragmentography. Of total urinary bufotenin a relatively constant amount, 59.9 - 69.0%, was excreted in conjugated form. The conjugate was tentatively identified as a glucuronide.
血清素的N，N - 二甲基化衍生物，蟾蜍色胺，以游离胺的形式排泄到正常人尿液中。然而，由于分子上的酚羟基，蟾蜍色胺的结合是可能的。在本研究中，检测了10名健康、未服用药物受试者尿液中游离和结合蟾蜍色胺的排泄情况。采用酸水解和酶水解从其结合物中释放出胺。通过同位素稀释质谱分析法进行定量。在总尿蟾蜍色胺中，相对恒定的量（59.9 - 69.0%）以结合形式排泄。该结合物初步鉴定为葡糖醛酸苷。
