Kovár J, Schneider J, Skurský L, Dubský H
Anal Biochem. 1984 Feb;137(1):74-9. doi: 10.1016/0003-2697(84)90349-x.
The proposed method for ethanol determination is based on the simultaneous oxidation of ethanol and reduction of nitrosodimethylaniline to a quinonediimine derivative in the presence of NAD and horse liver alcohol dehydrogenase. Quinonediimine formed in this reaction is coupled with salicylamide and the absorbance of the resulting blue indaniline dye is measured. The new method yields identical results when compared with the currently used methods for the determination of blood alcohol (i.e., with the conventional enzymatic method, Widmark's method, and gas chromatography). Its main advantage is high sensitivity, little consumption of both enzyme and coenzyme, and the measurement in the visible range of the spectrum.
所提出的乙醇测定方法是基于在烟酰胺腺嘌呤二核苷酸（NAD）和马肝醇脱氢酶存在的情况下，乙醇的同时氧化以及亚硝基二甲基苯胺还原为醌二亚胺衍生物。该反应中形成的醌二亚胺与水杨酰胺偶联，并测量所得蓝色吲达胺染料的吸光度。与目前用于测定血液酒精的方法（即传统酶法、维德马克法和气相色谱法）相比，新方法产生的结果相同。其主要优点是灵敏度高、酶和辅酶消耗少，以及在光谱的可见范围内进行测量。
