Berger G, Tiede M D, Breton J
Biochem Biophys Res Commun. 1984 May 31;121(1):47-54. doi: 10.1016/0006-291x(84)90686-7.
Reaction centers from Rhodopseudomonas sphaeroides R 26 have been isolated from a crude extract obtained by lauryldimethylamine oxide extraction of chromatophore membranes, by HPLC using a combination of surface-mediated and size exclusion chromatography. The eluted RCs exhibit a normal activity (t 1/2 of the back-reaction is 70 ms) and are recovered in good yield (over 50% based on the activity) and purity (based on the A 280 nm/A 800 nm = 1.30 +/- 0.05 ratio and the characteristic 3 polypeptides SDS-PAGE pattern). The elution time (5-10 mn) is about two orders of magnitude faster than for the classical purification techniques.
球形红假单胞菌R 26的反应中心是从通过月桂基二甲基氧化胺萃取色谱膜得到的粗提物中，利用表面介导和尺寸排阻色谱相结合的高效液相色谱法分离出来的。洗脱后的反应中心表现出正常活性（反向反应的半衰期为70毫秒），且回收率高（基于活性超过50%）、纯度高（基于280纳米吸光度/800纳米吸光度 = 1.30 +/- 0.05的比率以及特征性的3种多肽SDS - 聚丙烯酰胺凝胶电泳图谱）。洗脱时间（5 - 10分钟）比传统纯化技术快约两个数量级。
