Shalitin C, Vishlizky A
Biochim Biophys Acta. 1984 Jul 18;782(3):328-30. doi: 10.1016/0167-4781(84)90069-1.
A procedure has been developed for the isolation of intact yeast chromatin from yeast nuclei. Autodigestion of chromatin observed during nuclear preparation was inhibited by the addition of 5 mM spermidine. The procedure is useful for the analysis of proteins associated with yeast chromatin.
已开发出一种从酵母细胞核中分离完整酵母染色质的方法。在细胞核制备过程中观察到的染色质自消化通过添加5 mM亚精胺得到抑制。该方法对于分析与酵母染色质相关的蛋白质很有用。
