Seitz W R
Clin Biochem. 1984 Apr;17(2):120-5. doi: 10.1016/s0009-9120(84)90318-7.
Reagents required for reactions that produce chemiluminescence (CL) or bioluminescence (BL) may be coupled to antibodies or antigens and used as labels for immunoassay. Because methods based on CL and BL have very low detection limits, they have the potential to replace assays that currently employ radioisotopes as labels. The feasibility of several BL and CL labels has been demonstrated. To date, isoluminol derivatives have been most widely studied. Several steroid assays involving isoluminol labels have been reported, and labelled compound has been detected at levels approaching 10(17) moles. Acridinium ester-labelled compounds have also been detected at this level. In addition to systems in which the label is a reactant required for light, CL and BL can be used to analyze the amount of product generated by enzyme labels. This approach has also yielded very low detection limits. Systems have been developed using enzyme labels that catalyze formation of ATP which is then assayed by the firefly reaction or that catalyze formation of peroxide which is determined by either luminol or peroxyoxalate CL.
产生化学发光（CL）或生物发光（BL）反应所需的试剂可与抗体或抗原偶联，并用作免疫测定的标记物。由于基于CL和BL的方法检测限极低，它们有可能取代目前使用放射性同位素作为标记物的检测方法。几种BL和CL标记物的可行性已得到证实。迄今为止，异鲁米诺衍生物得到了最广泛的研究。已经报道了几种涉及异鲁米诺标记物的类固醇检测方法，并且已检测到标记化合物的水平接近10（-17）摩尔。吖啶酯标记的化合物也已在该水平被检测到。除了标记物是光产生所需反应物的系统外，CL和BL还可用于分析酶标记物产生的产物量。这种方法也产生了极低的检测限。已经开发出使用酶标记物的系统，这些酶标记物催化ATP的形成，然后通过萤火虫反应进行测定，或者催化过氧化物的形成，过氧化物由鲁米诺或过氧草酸酯CL测定。
