Buffington C K, Stentz F B, Kitabchi A E
Diabetes. 1984 Jul;33(7):681-5. doi: 10.2337/diab.33.7.681.
Cultured human fibroblasts represent an appropriate model for studying both insulin receptor interaction and hormone responsiveness. We have investigated the properties of the pyruvate dehydrogenase multi-enzyme complex (PDC) and have studied the effects of various concentrations of porcine and biosynthetic human insulin (BHI) on the activity of the enzyme. Under optimal conditions of the assay, both BHI and porcine insulin activated PDC in a dose-dependent fashion in which full activation of the enzyme was achieved with 10(-8) M insulin. The half-maximal concentration for porcine and human insulin was similar, occurring at the level of 5 X 10(-9) M for activation of the PDC of human fibroblasts. We conclude that the PDC of cultured human fibroblasts is activated by both human and porcine insulin at a comparable physiologic concentration. Human fibroblasts may therefore serve as a useful model to study insulin action in isolated human tissue.
培养的人成纤维细胞是研究胰岛素受体相互作用和激素反应性的合适模型。我们研究了丙酮酸脱氢酶多酶复合物（PDC）的特性，并研究了不同浓度的猪胰岛素和生物合成人胰岛素（BHI）对该酶活性的影响。在测定的最佳条件下，BHI和猪胰岛素均以剂量依赖性方式激活PDC，其中10（-8）M胰岛素可使该酶完全激活。猪胰岛素和人胰岛素的半数最大浓度相似，在激活人成纤维细胞PDC时为5×10（-9）M。我们得出结论，培养的人成纤维细胞的PDC在相当的生理浓度下可被人胰岛素和猪胰岛素激活。因此，人成纤维细胞可作为研究分离的人体组织中胰岛素作用的有用模型。
