将双曲钩端螺旋体血清型帕托克中色氨酸生物合成所需基因克隆至大肠杆菌中。
Cloning of a gene required for tryptophan biosynthesis from Leptospira biflexa serovar patoc into Escherichia coli.
作者信息
Yelton D B, Charon N W
出版信息
Gene. 1984 May;28(2):147-52. doi: 10.1016/0378-1119(84)90251-8.
A clone bank, consisting of approx. 8100 colonies, has been created for the spirochete Leptospira biflexa serovar patoc in Escherichia coli using pBR322 as the vector. One of these clones contains the genetic information needed to complement a defect in the trpE gene of E. coli. The information resides on a 20.5-kb plasmid designated pYC1, which carries a 16-kb insert consisting of three HindIII fragments. It does not complement defects in other genes needed for the biosynthesis of tryptophan in E. coli.
已使用pBR322作为载体，在大肠杆菌中构建了一个由约8100个菌落组成的克隆文库，用于双曲钩端螺旋体血清型帕托克螺旋体。这些克隆之一包含补充大肠杆菌trpE基因缺陷所需的遗传信息。该信息位于一个名为pYC1的20.5 kb质粒上，该质粒携带一个由三个HindIII片段组成的16 kb插入片段。它不能补充大肠杆菌中色氨酸生物合成所需的其他基因的缺陷。
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