Azzarone B, Mareel M, Billard C, Scemama P, Chaponnier C, Macieira-Coelho A
Int J Cancer. 1984 Jun 15;33(6):759-64. doi: 10.1002/ijc.2910330608.
The growth properties of fibroblasts from the thoracic skin of patients with mammary cancers were compared to those of fibroblastic cultures from patients with benign lesions or having undergone surgery for non-neoplastic diseases. As expected, an inverse correlation was found between the doubling potential of fibroblasts in vitro and the donor's age for cells from patients with benign lesions; however no correlation, was found with cultures from cancer patients. Moreover, the latter group responded in an abnormal way to three biological parameters: anchorage dependence, colony formation on monolayers of normal human epithelial cells and saturation densities in overcrowded culture conditions. Skin fibroblasts from one patient with a benign lesion, whose mother had developed a breast cancer, displayed all the abnormal growth properties. Periodic controls of this patient resulted in the early detection of a carcinoma 3 years after the first operation for a benign microcystic lesion. Finally, we found that multiple subcultivations in overcrowded culture conditions cause the selection of a fibroblastic cell subset with greater growth potential which, in the cell strain tested, could invade foreign tissue in vitro.
将乳腺癌患者胸部皮肤的成纤维细胞生长特性与良性病变患者或接受过非肿瘤性疾病手术患者的成纤维细胞培养物的生长特性进行了比较。正如预期的那样，对于良性病变患者的细胞，体外成纤维细胞的倍增潜力与供体年龄之间存在负相关；然而，在癌症患者的培养物中未发现相关性。此外，后一组对三个生物学参数的反应异常：贴壁依赖性、在正常人上皮细胞单层上的集落形成以及过度拥挤培养条件下的饱和密度。一名患有良性病变的患者的皮肤成纤维细胞，其母亲曾患乳腺癌，表现出所有异常生长特性。对该患者的定期检查导致在首次因良性微囊性病变手术3年后早期发现了癌症。最后，我们发现，在过度拥挤的培养条件下进行多次传代培养会选择具有更大生长潜力的成纤维细胞亚群，在所测试的细胞系中，该亚群在体外可侵袭异体组织。
