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人白细胞干扰素群体的大小和电荷异质性表征

Characterization of the size and charge heterogeneities of human leukocyte interferon populations.

作者信息

Lin L S, Wiranowska-Stewart M, Chudizio T, Stewart W E

出版信息

Arch Virol. 1978;56(3):269-72. doi: 10.1007/BF01317857.

DOI:10.1007/BF01317857
PMID:637729
Abstract

Human leukocyte interferons are separable into two size components by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and are separable into two charge-components by DEAE-BiGel A chromatography. However, each of the charge-components resolved by ion-exchange chromatography contained both size-components, when analysed by SDS-PAGE. Thus, there are more than two distinct molecular populations of human leukocyte interferons.

摘要

通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE),人白细胞干扰素可分离成两个大小不同的组分,通过二乙氨基乙基-琼脂糖凝胶A柱层析可分离成两个电荷不同的组分。然而,当用SDS-PAGE分析时,离子交换层析分离得到的每个电荷组分都包含两个大小组分。因此,人白细胞干扰素存在两种以上不同的分子群体。

相似文献

1
Characterization of the size and charge heterogeneities of human leukocyte interferon populations.人白细胞干扰素群体的大小和电荷异质性表征
Arch Virol. 1978;56(3):269-72. doi: 10.1007/BF01317857.
2
Nature of the molecular heterogeneity of human leukocyte interferon.人白细胞干扰素的分子异质性本质
J Virol. 1976 Aug;19(2):425-34. doi: 10.1128/JVI.19.2.425-434.1976.
3
Interferon: purification and initial characterization from human diploid cells.干扰素:从人二倍体细胞中纯化及初步特性鉴定
Proc Natl Acad Sci U S A. 1976 Feb;73(2):520-523. doi: 10.1073/pnas.73.2.520.
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Human leukocyte interferon: production, purification to homogeneity, and initial characterization.人白细胞干扰素:生产、纯化至均一性及初步特性鉴定
Proc Natl Acad Sci U S A. 1979 Feb;76(2):640-4. doi: 10.1073/pnas.76.2.640.
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Physico-chemical properties of interferon produced by a mixed leukocyte suspension.混合白细胞悬液产生的干扰素的物理化学性质
Acta Virol. 1977 Sep;21(5):359-64.
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Elimination of size and charge heterogeneities of human leukocyte interferons by chemical cleavage.通过化学裂解消除人白细胞干扰素的大小和电荷异质性
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4200-4. doi: 10.1073/pnas.74.10.4200.
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SDS-polyacrylamide gel electrophoresis of purified human leucocyte interferon and the antiviral and anticellular activities of the different interferon species.纯化的人白细胞干扰素的SDS-聚丙烯酰胺凝胶电泳及不同干扰素种类的抗病毒和抗细胞活性
J Gen Virol. 1980 Oct;50(2):441-6. doi: 10.1099/0022-1317-50-2-441.
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Characterization of the heterogeneous molecules of human interferons: differences in the cross-species antiviral activities of various molecular populations in human leukocyte interferons.人干扰素异质分子的特性:人白细胞干扰素中不同分子群体的种间抗病毒活性差异
J Gen Virol. 1978 Apr;39(1):125-30. doi: 10.1099/0022-1317-39-1-125.
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Purification and characterization of human leukocyte interferon components.人白细胞干扰素成分的纯化与特性分析
J Biol Chem. 1976 Aug 25;251(16):4810-6.
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Human leukocyte interferon: separation of biologically different species by modification of carbohydrate moieties.人白细胞干扰素:通过碳水化合物部分的修饰分离生物学上不同的种类。
Arch Virol. 1980;63(2):133-42. doi: 10.1007/BF01320770.

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本文引用的文献

1
Nature of the molecular heterogeneity of human leukocyte interferon.人白细胞干扰素的分子异质性本质
J Virol. 1976 Aug;19(2):425-34. doi: 10.1128/JVI.19.2.425-434.1976.
2
Molecular heterogeneity of human leukocyte interferon: two populations differing in molecular weights, requirements for renaturation, and cross-species antiviral activity.人白细胞干扰素的分子异质性:存在两种分子量、复性要求及种间抗病毒活性不同的群体。
Virology. 1975 Sep;67(1):68-73. doi: 10.1016/0042-6822(75)90403-1.