Aronson D L, Franza B R, Bagley J
Thromb Res. 1984 Jun 1;34(5):419-29. doi: 10.1016/0049-3848(84)90246-9.
The activation of the prothrombin intermediate, Prethrombin 2, has been studied in order to establish test systems that would enable identification of Prethrombin 2 in serum and Factor IX concentrates. While activation of Prethrombin 2 by Taipan Snake Venom (TSV) was slow and incomplete, inclusion of approximately molar amounts of prothrombin fragments F1 or F1.2 markedly enhanced the amount of thrombin formed by TSV. This effect could also be obtained by the inclusion of serum. Neither normal serum nor Factor V deficient serum contain any identifiable Prethrombin 2. On the other hand substantial amounts of Prethrombin 2 are present in Factor IX concentrates used for the treatment of Christmas Disease (Hemophilia B).
为了建立能够识别血清和凝血因子IX浓缩物中凝血酶原中间体（凝血酶原2）的检测系统，对凝血酶原2的激活进行了研究。虽然太攀蛇毒（TSV）对凝血酶原2的激活缓慢且不完全，但加入大约摩尔量的凝血酶原片段F1或F1.2可显著提高TSV形成的凝血酶量。加入血清也可获得这种效果。正常血清和缺乏凝血因子V的血清中均未检测到任何可识别的凝血酶原2。另一方面，用于治疗血友病B（克里斯马斯病）的凝血因子IX浓缩物中存在大量的凝血酶原2。
