[大肠杆菌α-溶血素的特性]

[Properties of Escherichia coli alpha-hemolysin].

作者信息

Favorov V V, Sprygin V G, Atrashok R I, Abramov A K, Khavilov A I

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1984 May(5):44-8.

Abstract

SH-reagents (alpha-ethyl maleimide, n-chlor mercuribenzoate, dithioglycolic acid) and hydrogen peroxide induce insignificant changes in the activity of hemolysin. Reducing reagents (2-mercaptoethanol and ascorbate) inhibit hemolytic activity. Cholesterol at a concentration of 4.3 X 10(-5) M reduces this activity by 50%. Hemolysin has no phospholipase A activity. The energy necessary for activating the interaction of hemolysin with the membranes of erythrocytes (lag period) is 13800 cal/mol and for inducing the lysis of erythrocytes, 10 600 cal/mol. The above values are much less than those of O-labile hemolysins.

摘要

巯基试剂（α-乙基马来酰亚胺、对氯汞苯甲酸、二硫代乙醇酸）和过氧化氢对溶血素活性的影响不显著。还原试剂（2-巯基乙醇和抗坏血酸盐）会抑制溶血活性。浓度为4.3×10⁻⁵ M的胆固醇可使该活性降低50%。溶血素没有磷脂酶A活性。激活溶血素与红细胞膜相互作用（延迟期）所需的能量为13800卡/摩尔，诱导红细胞裂解所需的能量为10600卡/摩尔。上述数值远低于对氧不稳定的溶血素的数值。