Sumegi B, Srere P A
J Biol Chem. 1984 Jul 25;259(14):8748-52.
The binding of crotonase (enoyl-CoA hydratase), beta-hydroxyacyl-CoA dehydrogenase, beta-ketothiolase, succinyl-CoA transferase, and carnitine acetyltransferase to inner mitochondrial membranes, mitoplasts, intact mitochondria, erythrocyte membranes, and phosphatidylcholine liposomes was studied. Succinyl-CoA transferase does not bind to any of these membranes. Carnitine acetyltransferase, on the other hand, binds to all of these membranes. The enzymes of fatty acid beta-oxidation, thiolase, crotonase, and beta-hydroxyacyl-CoA dehydrogenase bind to inner membrane, but not to liposomes. The binding shows a moderate dependence on ionic strength (2-200 mM) and pH (6.5-8). These data indicate the possibility of an organization of the enzymes of fatty acid beta-oxidation on the inner mitochondrial membrane, but do not support the idea of an organization of the enzymes of ketone body catabolism.
研究了巴豆酸酶（烯酰辅酶A水合酶）、β-羟酰基辅酶A脱氢酶、β-酮硫解酶、琥珀酰辅酶A转移酶和肉碱乙酰转移酶与线粒体内膜、线粒体膜、完整线粒体、红细胞膜和磷脂酰胆碱脂质体的结合情况。琥珀酰辅酶A转移酶不与这些膜中的任何一种结合。另一方面，肉碱乙酰转移酶与所有这些膜结合。脂肪酸β-氧化的酶，硫解酶、巴豆酸酶和β-羟酰基辅酶A脱氢酶与内膜结合，但不与脂质体结合。这种结合对离子强度（2 - 200 mM）和pH（6.5 - 8）有适度的依赖性。这些数据表明脂肪酸β-氧化的酶有可能在线粒体内膜上形成组织，但不支持酮体分解代谢的酶形成组织的观点。
