Ekström P, Van Veen T
J Comp Neurol. 1984 Jul 1;226(3):307-20. doi: 10.1002/cne.902260302.
The distributions of serotoninergic neurons in the brain of the three-spined stickleback was demonstrated with the indirect peroxidase-antiperoxidase (PAP) immunohistochemical method with antibodies against serotonin. Serotoninergic perikarya were demonstrated in the brainstem reticular formation (nucleus raphe dorsalis, nucleus raphe medialis, and nucleus tegmenti dorsalis lateralis) and in the periventricular ventral thalamus and hypothalamus (nucleus ventromedialis thalami, nucleus posterioris periventricularis, nucleus recessus lateralis, and nucleus recessus posterioris). After pharmacological pretreatment of the animals with a monoamine oxidase inhibitor, serotoninergic perikarya were also visualized in area praetectalis and in the medial brainstem, caudal to nucleus raphe medialis. Whereas the cell groups of the brainstem give rise to both ascending and descending pathways, it was not possible to analyze the distribution of efferent projections from the diencephalic cell groups. Distribution of serotoninergic axons showed marked regional differences. Only scattered varicose fibers were demonstrated in the cerebellum, the facial lobes, and the lateral line lobes. In the mesencephalon, the dorsal periventricular tegmentum and the central gray receive only small numbers of serotoninergic axons, while torus semicircularis and the visual layers of tectum opticum are profusely innervated. In the diencephalon, the hypothalamus and ventral thalamus generally display the highest density of serotoninergic axons. Exceptions are found in nucleus glomerulosus and the ventromedial portion of lobus inferioris, where densities are low. In the telencephalon, the density of serotoninergic axons is very high in area dorsalis pars medialis and pars lateralis dorsalis, but low in area dorsalis pars dorsalis and pars lateralis ventralis, and intermediate in area ventralis.
采用抗血清素抗体的间接过氧化物酶-抗过氧化物酶（PAP）免疫组织化学方法，证实了三刺鱼脑中血清素能神经元的分布。血清素能神经元胞体见于脑干网状结构（中缝背核、中缝内侧核和外侧被盖背核）以及室周腹侧丘脑和下丘脑（丘脑腹内侧核、室周后核、外侧隐窝核和后隐窝核）。在用单胺氧化酶抑制剂对动物进行药理学预处理后，在视前区和中脑内侧、中缝内侧核尾侧也可见到血清素能神经元胞体。虽然脑干的细胞群产生了上行和下行通路，但无法分析间脑细胞群传出投射的分布。血清素能轴突的分布显示出明显的区域差异。在小脑、面叶和侧线叶仅见散在的曲张纤维。在中脑，室周背侧被盖和中央灰质仅接受少量血清素能轴突，而半环形隆凸和视顶盖的视觉层则有丰富的神经支配。在间脑，下丘脑和腹侧丘脑通常显示出血清素能轴突的最高密度。在肾小球核和下叶内侧部分密度较低，属于例外情况。在端脑，血清素能轴突密度在背侧内侧部和背侧外侧部非常高，但在背侧背侧部和腹侧外侧部较低，在腹侧部则居中。
