Gubnitskiĭ L S, Mekhtiev A A, Iakovleva V I, Berezin I V
Prikl Biokhim Mikrobiol. 1984 May-Jun;20(3):329-33.
The fumarate hydratase activity of intact cells was determined for 36 strains of Escherichia coli, receiver from the All-Union Collection of Microorganisms, to reveal a producer of L-malic acid. A research was made to find optimal media for cultivating microorganisms possessing the fumarate hydratase activity. Spectrophotometric and chromatographic methods were chosen to detect malic acid in the complete reaction mixture, which are available for kinetic study of the malic acid synthesis from potassium fumarate.
测定了从全苏微生物保藏中心获得的36株大肠杆菌完整细胞的富马酸水合酶活性，以找出L-苹果酸的产生菌。开展研究以寻找用于培养具有富马酸水合酶活性的微生物的最佳培养基。选择分光光度法和色谱法来检测完全反应混合物中的苹果酸，这些方法可用于对由富马酸钾合成苹果酸的动力学研究。
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