Hol P R, Langeveld J P, van Beuningen-Jansen E W, Veerkamp J H, Gruys E
Scand J Immunol. 1984 Jul;20(1):53-60. doi: 10.1111/j.1365-3083.1984.tb00977.x.
Amyloid fibrils were isolated from the renal papillae and glomeruli of cows with spontaneous AA amyloidosis. The fibrils were solubilized by treatment with guanidine hydrochloride (Gu HCl) and subjected to gel filtration on Sephacryl S-200. Two other fractions were obtained beside the void volume and the AA fractions. Reaggregation studies were performed by dialysing the fractions, separately or in combinations, against Gu-HCl-free solutions. Protein AA alone (about 10 kd) appeared not to precipitate. The other fractions alone and the combinations of fractions tested formed precipitates. The precipitates containing all fractions (including protein AA) or protein AA plus a fraction containing a 19- and a 23-kd protein revealed congophilic green birefringent fibrillar material. Dialysis against acidic and calcium-containing solutions gave the best results. Amyloid fibril-like material was visible on electron microscopic examination. The amino acid composition of the 19 + 23-kd material appeared to be slightly different from protein AA and evidently unlike SAP. On immunofluorescence-absorbance studies the 19 + 23-kd material appeared evidently unlike protein AA and SAP. From these findings it is concluded that for spontaneous formation of AA amyloid fibrils other non-AA proteins are necessary.
从患有自发性AA淀粉样变性的奶牛的肾乳头和肾小球中分离出淀粉样纤维。通过用盐酸胍（Gu HCl）处理使纤维溶解，并在Sephacryl S-200上进行凝胶过滤。除了空体积和AA组分外，还获得了另外两个组分。通过将这些组分单独或组合地与不含Gu-HCl的溶液进行透析来进行再聚集研究。单独的蛋白质AA（约10 kd）似乎不会沉淀。单独的其他组分以及测试的组分组合形成沉淀。含有所有组分（包括蛋白质AA）或蛋白质AA加上含有19 kd和23 kd蛋白质的组分的沉淀物显示出嗜刚果红绿色双折射纤维状物质。对酸性和含钙溶液进行透析得到了最好的结果。在电子显微镜检查中可见淀粉样纤维样物质。19 + 23 kd物质的氨基酸组成似乎与蛋白质AA略有不同，显然也与血清淀粉样蛋白P成分（SAP）不同。在免疫荧光吸收研究中，19 + 23 kd物质显然与蛋白质AA和SAP不同。从这些发现可以得出结论，对于AA淀粉样纤维的自发形成，其他非AA蛋白质是必需的。
