Hunter N R, Taylor G R, Swank P R, Winkler D G, Thompson J L, Montalvo J, Greenberg S D, McFadyen G M, Estrada R
Anal Quant Cytol. 1984 Jun;6(2):139-43.
Two methods for precise cell location and relocation were developed at the Cell Image Analysis Laboratory, NASA, to increase the efficiency of the cell image acquisition process. The first method uses a Zeiss Zonax with a 10 micron scanning stage and automatically records cell coordinates on 5.25-inch floppy disks. The second method uses a Digital Positioning Device with a 10 micron scanning stage, and the cell coordinates displayed on light-emitting diodes (LEDs) are recorded manually. Because of its superior speed and automation, the Zonax was selected as the method of choice. By using a Zonax for off-line cell location and another Zonax for on-line cell relocation, more than three times the number of digitized images were collected in ten months than in the previous three years. Other beneficial applications of the cell location and relocation methods were realized in photomicrography, data base verification and cytopathology.
美国国家航空航天局细胞图像分析实验室开发了两种精确的细胞定位和重新定位方法，以提高细胞图像采集过程的效率。第一种方法使用配备10微米扫描台的蔡司Zonax，并自动将细胞坐标记录在5.25英寸软盘上。第二种方法使用配备10微米扫描台的数字定位设备，手动记录发光二极管（LED）上显示的细胞坐标。由于其卓越的速度和自动化程度，Zonax被选为首选方法。通过使用一台Zonax进行离线细胞定位，另一台Zonax进行在线细胞重新定位，十个月内采集的数字化图像数量比前三年多了三倍多。细胞定位和重新定位方法在显微摄影、数据库验证和细胞病理学中也有其他有益的应用。
