Mørk S J
Anticancer Res. 1984 May-Jun;4(3):189-95.
In long term culture of N-ethyl-N-nitrosourea (ENU) exposed fetal BD IX-rat brains, cells proliferate and form aggregates (nodules) in vitro, starting 2-3 months after the transplacental carcinogen injection in vivo. Cell nodules of cultures from 3-6 months after ENU exposure were investigated for the presence of organoid structures, signs of differentiation, and differentiation products. Organoid structures were absent as were signs of differentiation into more mature neuroglial cells since a relatively uniform immature cell population was typical for the nodules studied. Nuclear changes in form of nuclear envelope dilatation, margination and condensation of chromatin were present. The S-100 protein was generally expressed by the neuroglial cells in this premalignant phase; GFA and 14.3.2. protein were expressed to a variable degree. The cells showed enhanced proliferation. In conclusion there was a gradual change of differentiation accompanied by the acquisition of atypical nuclear structure in the premalignant phase studied.
在经N-乙基-N-亚硝基脲（ENU）处理的BD IX系大鼠胎脑的长期培养中，在经胎盘注射致癌剂后的2至3个月开始，细胞在体外增殖并形成聚集体（结节）。对ENU暴露后3至6个月培养物中的细胞结节进行了研究，以确定是否存在类器官结构、分化迹象和分化产物。由于所研究的结节中典型的是相对均匀的未成熟细胞群体，因此不存在类器官结构，也没有分化为更成熟神经胶质细胞的迹象。存在核膜扩张、染色质边缘化和凝聚形式的核变化。在这个癌前阶段，神经胶质细胞通常表达S-100蛋白；GFA和14.3.2.蛋白表达程度不一。细胞显示出增殖增强。总之，在所研究的癌前阶段，伴随着非典型核结构的获得，分化逐渐发生变化。
