Hoff H F, Bond M G
Artery. 1983;12(2):104-16.
The presence of apolipoprotein B (apo B) was determined in atherosclerotic lesions of coronary arteries from cynomolgus monkeys placed on a hypercholesterolemic diet for one year. Immunofluorescence techniques were applied to sections of arteries that were fixed by perfusion with formalin and embedded in paraffin. Segments of right coronary, left anterior descending, and left circumflex arteries were observed. All segments demonstrated substantial intimal thickening, accompanied by areas of necrosis at the base of the plaque, usually located on the luminal side of the internal elastic lamina. Large clusters of foam cells could be found at the base of such plaques, between intimal smooth muscle cells in the fibromuscular cap, and frequently in the tunica media in regions of necrosis. Immunofluorescence depicting apo B was usually confined to the intima, especially in the necrotic core, but could also be seen in the media in regions in which the internal elastic lamina was missing. Apo B often filled the extracellular space between smooth muscle cells and groups of foam cells but was not identified in the cytoplasm of intact cells. Apo B-positive areas were usually also positive for alcian blue staining, suggesting LDL-sulfated glycosaminoglycan coexistence at such sites. The localization pattern of apo B in lesioned coronary arteries from cynomolgus monkeys was consistent with that found in human coronary lesions, suggesting that diet-induced hypercholesterolemia in such monkeys may be a valid model for studying the role of LDL in human atherogenesis.
对食高胆固醇饮食一年的食蟹猴冠状动脉粥样硬化病变中载脂蛋白B（apo B）的存在情况进行了测定。免疫荧光技术应用于经福尔马林灌注固定并石蜡包埋的动脉切片。观察了右冠状动脉、左前降支和左旋支动脉的节段。所有节段均显示出明显的内膜增厚，并伴有斑块底部的坏死区域，通常位于内弹性膜的管腔侧。在这类斑块的底部、纤维肌帽的内膜平滑肌细胞之间以及坏死区域的中膜中经常可以发现大量泡沫细胞簇。显示apo B的免疫荧光通常局限于内膜，尤其是在坏死核心，但在缺少内弹性膜的区域的中膜中也可见。apo B常常充满平滑肌细胞与泡沫细胞群之间的细胞外间隙，但在完整细胞的细胞质中未发现。apo B阳性区域通常对阿尔辛蓝染色也呈阳性，表明在此类部位存在低密度脂蛋白 - 硫酸化糖胺聚糖共存。食蟹猴病变冠状动脉中apo B的定位模式与人类冠状动脉病变中的情况一致，这表明此类猴子的饮食诱导性高胆固醇血症可能是研究低密度脂蛋白在人类动脉粥样硬化发生中作用的有效模型。
