Hogan J J, Gutell R R, Noller H F
Biochemistry. 1984 Jul 3;23(14):3330-5. doi: 10.1021/bi00309a033.
The conformation and accessibility of 26S rRNA in yeast 60S ribosomal subunits were probed with kethoxal. Oligonucleotides originating from reactive sites were isolated by diagonal electrophoresis and sequenced. From over 70 oligonucleotide sequences, 26 kethoxal-reactive sites could be placed in the 26S rRNA sequence. These are in close agreement with a proposed secondary structure model for the RNA that is based on comparative sequence analysis. At least seven kethoxal-reactive sites in yeast 26S rRNA are in positions that are exactly homologous to reactive positions in E. coli 23S rRNA; each of these sites has previously been implicated in some aspect of ribosomal function.
用乙二醛探测酵母60S核糖体亚基中26S rRNA的构象和可及性。通过对角线电泳分离源自反应位点的寡核苷酸并进行测序。从70多个寡核苷酸序列中，可将26个乙二醛反应位点置于26S rRNA序列中。这些与基于比较序列分析提出的RNA二级结构模型高度一致。酵母26S rRNA中至少有七个乙二醛反应位点与大肠杆菌23S rRNA中的反应位点位置完全同源；这些位点中的每一个先前都与核糖体功能的某些方面有关。
