Nakatani T, Sato T, Marzella L, Hirai F, Trump B F, Siegel J H
Circ Shock. 1984;13(3):271-94.
A single, uniform abscess was formed in 100% of the animals inoculated with a fecal pellet made of sterile rat feces, agar, and a known number and strain of bacteria. The effects of monoclonal Escherichia coli abscess (83 rats) were compared to those of sterile abscess (34 rats) and sham operation (35 rats without abscess). Bacteroides fragilis was added to the sterile pellet to study the effect of an anaerobic monoclonal abscess (16 rats) or of a biclonal abscess containing both aerobes and anaerobes (32 rats). After inoculation, a peritonitis stage with leucopenia, hypoglycemia, body weight loss, and slight fever was followed by the abscess stage with leucocytosis and a slight hyperglycemia. Mild hepatic energy charge deficiency and hepatic lactic acidosis were observed in sterile abscess rats, and slightly enhanced energy charge was seen in monoclonal E. coli abscess rats. The addition of B. fragilis to the sterile pellet, alone or together with E. coli, produced hepatic energy charge deficiency and hepatic lactic acidosis, which were significantly enhanced compared with the monoclonal E. coli abscess rats. The greatest effect was seen in the biclonal E. coli plus B. fragilis abscess, suggesting that anaerobic or combined aerobe and anaerobe abscesses may produce a greater hepatic injury than an aerobic organism abscess alone. This may account for the apparent synergic interaction between aerobic and anaerobic organisms.
在接种由无菌大鼠粪便、琼脂以及已知数量和菌株的细菌制成的粪便颗粒的所有动物中，均形成了单个、均匀的脓肿。将单克隆大肠杆菌脓肿组（83只大鼠）的效果与无菌脓肿组（34只大鼠）和假手术组（35只无脓肿大鼠）进行了比较。向无菌颗粒中添加脆弱拟杆菌，以研究厌氧单克隆脓肿（16只大鼠）或包含需氧菌和厌氧菌的双克隆脓肿（32只大鼠）的效果。接种后，先是出现伴有白细胞减少、低血糖、体重减轻和低热的腹膜炎阶段，随后是伴有白细胞增多和轻度高血糖的脓肿阶段。在无菌脓肿大鼠中观察到轻度肝能荷不足和肝乳酸酸中毒，而在单克隆大肠杆菌脓肿大鼠中可见能荷略有增强。向无菌颗粒中单独添加脆弱拟杆菌或与大肠杆菌一起添加，均会导致肝能荷不足和肝乳酸酸中毒，与单克隆大肠杆菌脓肿大鼠相比显著增强。在双克隆大肠杆菌加脆弱拟杆菌脓肿中观察到的影响最大，这表明厌氧或需氧菌与厌氧菌混合的脓肿可能比单纯需氧菌脓肿造成更大的肝损伤。这可能解释了需氧菌和厌氧菌之间明显的协同相互作用。
