Holmberg V, Wahren B, Esposti P L
Cytometry. 1984 Jul;5(4):437-41. doi: 10.1002/cyto.990050423.
A peroxidase-antiperoxidase (PAP) technique was developed for the detection of carcinoembryonic antigen (CEA) in urothelial transitional cells of 52 bladder cancer patients. The percentage of CEA-containing malignant cells varied from 10% to 100%. As a mean, 65% of the malignant cells stained for CEA, while the corresponding figure for benign-looking cells was 24%. The results were compared with cytological evaluations, flow cytophotometric results, and immunofluorescent (IF) staining for CEA. With increasing malignancy, more CEA was detected with the PAP technique, whereas the IF technique failed to show this trend. 18 of 20 malignant-tumors had an aneuploid DNA pattern. The two diploid cases were moderately well differentiated. Samples from bladders with heavy inflammation should be avoided in the PAP technique, since the unspecific staining of granulocytes disturbed a correct evaluation of the transitional cells. The PAP technique used on cytological material is recommended for antigen determinations, since good morphology is obtained.
采用过氧化物酶-抗过氧化物酶（PAP）技术检测52例膀胱癌患者尿路上皮移行细胞中的癌胚抗原（CEA）。含CEA的恶性细胞百分比在10%至100%之间。平均而言，65%的恶性细胞CEA染色呈阳性，而外观正常细胞的相应比例为24%。将结果与细胞学评估、流式细胞光度测定结果以及CEA免疫荧光（IF）染色结果进行比较。随着恶性程度增加，PAP技术检测到更多的CEA，而IF技术未能显示出这种趋势。20例恶性肿瘤中有18例具有非整倍体DNA模式。两例二倍体病例分化程度为中等。在PAP技术中应避免使用炎症严重的膀胱样本，因为粒细胞的非特异性染色干扰了对移行细胞的正确评估。由于能获得良好的形态，建议在细胞学材料上使用PAP技术进行抗原测定。
