Kelley C G, Yamaguchi T, Santana E, Kaufman H E
Invest Ophthalmol Vis Sci. 1984 Sep;25(9):1061-4.
Human donor corneas were used for penetrating keratoplasty in one eye of each of 12 rhesus monkeys. In six animals, a 9.5-mm cornea was sutured into a 9.0-mm recipient bed by means of interrupted 10-0 nylon sutures. Six other animals received a 6.5-mm cornea in a 6.0-mm bed. Biomicroscopy, pachymetry, and specular microscopy revealed two distinct healing patterns. Of the six eyes receiving the smaller grafts, five showed prompt, stable clearing and thinning of the grafts with endothelial cell densities ranging from 850 to 1600 cells/mm2 Two of the six animals receiving larger grafts developed fibrinous reactions in the immediate postoperative period, and the grafts never cleared. Three showed a satisfactory early course, but after 10-16 days, developed endothelial keratic precipitates, anterior chamber reaction, and progressive graft edema. The sixth graft remained technically satisfactory 1 year later. This study indicates that the application of small human donor grafts in monkey eyes can provide a useful, clinical model for the future exploration of the response of human corneal transplants to materials such as epidermal growth factor and for the study of surgical manipulation of postkeratoplasty astigmatism.
人类供体角膜被用于12只恒河猴每只的一只眼睛的穿透性角膜移植手术。在6只动物中，通过间断的10-0尼龙缝线将9.5毫米的角膜缝合到9.0毫米的受体床中。另外6只动物在6.0毫米的床中接受了6.5毫米的角膜。生物显微镜检查、测厚法和镜面显微镜检查揭示了两种不同的愈合模式。在接受较小移植物的6只眼中，5只显示移植物迅速、稳定地清亮和变薄，内皮细胞密度范围为850至1600个细胞/平方毫米。接受较大移植物的6只动物中有2只在术后即刻出现纤维蛋白反应，移植物从未清亮。3只显示早期过程令人满意，但在10 - 16天后，出现内皮角膜后沉着物、前房反应和进行性移植物水肿。第六只移植物在1年后技术上仍令人满意。这项研究表明，在猴眼中应用小型人类供体移植物可为未来探索人类角膜移植对表皮生长因子等材料的反应以及研究角膜移植术后散光的手术操作提供一个有用的临床模型。
