Ueda H, Fujimoto S
J UOEH. 1984 Mar 1;6(1):39-46. doi: 10.7888/juoeh.6.39.
By the application of the protein A-gold technique for immunocytochemistry of the tilapia pituitary growth hormone (GH), our electron micrographs revealed that the antigenicity of tilapia GH and the cytoplasmic structure were well preserved in the tissues fixed with periodate-lysine-paraformaldehyde followed by osmium tetroxide postfixation. Using the specific antiserum against tilapia GH, the immunoreactive gold particles of 20-40 nm in size were located on secretory granules (200-300 nm in diameter) exclusively of one cell type in the proximal pars distalis identified as GH cells on the basis of their ultrastructural characteristics. This technique is considered to be fairly efficient for the detection of intracellular antigenic sites on ultrathin sections.
通过应用蛋白质A-金技术对罗非鱼垂体生长激素（GH）进行免疫细胞化学研究，我们的电子显微镜照片显示，在用高碘酸盐-赖氨酸-多聚甲醛固定并经四氧化锇后固定的组织中，罗非鱼GH的抗原性和细胞质结构得到了很好的保存。使用针对罗非鱼GH的特异性抗血清，大小为20 - 40纳米的免疫反应性金颗粒仅位于远侧部近端一种细胞类型的分泌颗粒（直径200 - 300纳米）上，根据其超微结构特征，该细胞类型被鉴定为GH细胞。该技术被认为在超薄切片上检测细胞内抗原位点方面相当有效。
