Ziegler M, Dietz H, Keilacker H, Witt S, Ziegler B
Biomed Biochim Acta. 1984;43(5):695-701.
Mouse monoclonal antibodies were raised against human insulin by somatic cell hybridization using the mouse myeloma line P3-X63-Ag8. Five cell lines were grown as ascites producing tumors. Insulin binding data were determined from six monoclonal antibodies by Scatchard analysis. Each anti-insulin hybridoma antibody gave a straight-line Scatchard plot confirming the homogeneity of their binding sites and their monoclonality. The equilibrium dissociation constants ranged from 2.20 X 10(-8) to 4.48 X 10(-10) mol/l. We could demonstrate positive as well as negative cooperativity of monoclonal insulin antibodies by performing insulin binding studies with defined mixtures of two different anti-insulin hybridoma antibodies.
利用小鼠骨髓瘤细胞系P3-X63-Ag8，通过体细胞杂交制备了抗人胰岛素的小鼠单克隆抗体。五个细胞系培养成产生腹水的肿瘤。通过Scatchard分析从六种单克隆抗体中测定胰岛素结合数据。每种抗胰岛素杂交瘤抗体均给出直线型Scatchard图，证实了其结合位点的同质性及其单克隆性。平衡解离常数范围为2.20×10⁻⁸至4.48×10⁻¹⁰mol/L。通过用两种不同抗胰岛素杂交瘤抗体的特定混合物进行胰岛素结合研究，我们可以证明单克隆胰岛素抗体存在正协同性和负协同性。
