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[不同角膜保存方法的应用可能性及生物学特性]

[Possibilities and biological properties of variously applied methods of corneal preservation].

作者信息

Böhnke M, Draeger J, Winter R

出版信息

Klin Monbl Augenheilkd. 1984 Aug;185(2):91-4. doi: 10.1055/s-2008-1054576.

Abstract

As the demand for donor corneas increases, not only fresh donor material but also increasing numbers of preserved corneas are being transplanted. The simple eye-banking procedures such as the moist chamber and the McCarey-Kaufman medium provide sufficient endothelial cell viability for short periods. The methods of organ culture of corneoscleral disks extend storage periods to up to four weeks. Cryopreservation as a method of long-term storage has not come to be widely used owing to the complicated procedure involved and the considerable damage thus caused to endothelial cells. Thus, the organ culture method may represent a compromise between extended storage periods and good endothelial cell viability.

摘要

随着对供体角膜需求的增加,不仅新鲜的供体材料,而且越来越多的保存角膜也被用于移植。诸如湿房和麦卡里-考夫曼培养基等简单的眼库程序能在短时间内提供足够的内皮细胞活力。角膜缘组织块器官培养方法可将保存时间延长至四周。由于冷冻保存方法涉及的程序复杂且会对内皮细胞造成相当大的损伤,尚未得到广泛应用。因此,器官培养方法可能是延长保存时间和保持良好内皮细胞活力之间的一种折衷办法。

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