Cornell H J, Boughdady N M, Herington A C
Prep Biochem. 1984;14(2):123-38. doi: 10.1080/10826068408070619.
Insulin-like growth factors I and II have been isolated from Cohn fraction IV-1 of human plasma using gel permeation chromatography, ion exchange chromatography, reversed phase chromatography, isoelectric focusing (IEF) and high performance liquid chromatography(HPLC). IGF I of specific activity 89 U/g, as measured by the isolated rat adipocyte assay, and IGF II, of specific activity 78 U/g, were obtained in yields of 16 micrograms and 34 micrograms respectively per 100g of Cohn fraction. Although this process yields IGF I which is contaminated with IGF II (due to the relatively large amount of the latter present in the original plasma), the IGF II preparations appear to be relatively free from IGF I. This separation was mainly achieved with IEF since the two factors elute close together on HPLC. Nevertheless, HPLC is important for their subsequent purification. The process is thus especially suitable for the preparation of IGF II and appears to give better yields than those obtained by earlier methods which used acid-ethanol extraction, gel permeation chromatography and polyacrylamide gel electrophoresis.
利用凝胶渗透色谱法、离子交换色谱法、反相色谱法、等电聚焦（IEF）和高效液相色谱法（HPLC），已从人血浆的科恩IV-1组分中分离出胰岛素样生长因子I和II。通过分离的大鼠脂肪细胞测定法测得，比活性为89 U/g的IGF I和比活性为78 U/g的IGF II，每100g科恩组分的产量分别为16微克和34微克。尽管此过程产生的IGF I被IGF II污染（由于原始血浆中存在相对大量的后者），但IGF II制剂似乎相对不含IGF I。这种分离主要通过IEF实现，因为这两种因子在HPLC上洗脱位置接近。然而，HPLC对它们随后的纯化很重要。因此，该方法特别适合制备IGF II，并且似乎比早期使用酸乙醇提取、凝胶渗透色谱法和聚丙烯酰胺凝胶电泳的方法获得的产量更高。
