Cornell H J, Brady P H
Department of Applied Chemistry, Royal Melbourne Institute of Technology, Australia.
J Chromatogr. 1987 Oct 9;421(1):61-9. doi: 10.1016/0378-4347(87)80379-1.
Substantially purified insulin-like growth factor II (IGF-II) was prepared from human serum. Initial enrichment using ion-exchange chromatography on DEAE Sephadex A50, followed by gel permeation chromatography on Sephadex G-75 in 1% formic acid produced material suitable for application to a preparative reversed-phase high-performance liquid chromatographic (HPLC) column containing LiChroprep RP-18. The latter step gave about 90-fold purification with a recovery of about 70% IGF-II bio-activity. Finally, a small reversed-phase HPLC column achieved a 17-fold purification with similar yield of activity. Overall, the four steps gave IGF-II of about 90% purity in yield of 12%.
从人血清中制备了高度纯化的胰岛素样生长因子II（IGF-II）。首先在DEAE Sephadex A50上进行离子交换色谱初步富集，然后在1%甲酸中的Sephadex G-75上进行凝胶渗透色谱，得到适用于含LiChroprep RP-18的制备型反相高效液相色谱（HPLC）柱的材料。后一步实现了约90倍的纯化，IGF-II生物活性回收率约为70%。最后，一个小型反相HPLC柱实现了17倍的纯化，活性产率相似。总体而言，这四个步骤得到了纯度约为90%的IGF-II，产率为12%。
