神经节苷脂GD2和GD3在黏着斑及人黑色素瘤细胞表面的定位。

Cheresh D A, Harper J R, Schulz G, Reisfeld R A

Proc Natl Acad Sci U S A. 1984 Sep;81(18):5767-71. doi: 10.1073/pnas.81.18.5767.

The predominant gangliosides produced by two cultured human melanoma cell lines are GD3 and/or GD2. These gangliosides were found to be cell associated and present in substratum-attached material after cell removal by EDTA. Monoclonal antibodies directed to GD2 and GD3 specified the cell-surface distribution of these gangliosides and localized them in focal adhesion plaques at the interface of cells and their substratum. These attachment sites did not represent indiscriminant membrane fragments remaining after removal of cells with EDTA, because neither melanoma-associated proteoglycan nor class I histocompatibility antigens were detected by their respective antibodies. Our data suggest that the disialogangliosides GD2 and GD3 may be involved in the interaction between human melanoma cells and solid substrata.

两种培养的人黑色素瘤细胞系产生的主要神经节苷脂是GD3和/或GD2。这些神经节苷脂被发现与细胞相关，并在通过EDTA去除细胞后存在于附着在基质上的物质中。针对GD2和GD3的单克隆抗体确定了这些神经节苷脂在细胞表面的分布，并将它们定位在细胞与其基质界面处的粘着斑中。这些附着位点并不代表用EDTA去除细胞后残留的随意膜片段，因为用它们各自的抗体未检测到黑色素瘤相关蛋白聚糖或I类组织相容性抗原。我们的数据表明，二唾液酸神经节苷脂GD2和GD3可能参与人黑色素瘤细胞与固体基质之间的相互作用。