Newbrun E, Matsukubo T, Hoover C I, Graves R C, Brown A T, Disney J A, Bohannan H M
Community Dent Oral Epidemiol. 1984 Oct;12(5):325-31. doi: 10.1111/j.1600-0528.1984.tb01464.x.
Two simple microbiological tests for quantitating salivary Streptococcus mutans levels were compared with each other and evaluated for suitability for mass screenings and private practice. Both tests use mitis salivarius medium with bacitracin (MSB) and are selective for S. mutans. One test estimates colonies grown on agar (MSBA) and the other estimates colonies grown in broth that adhere to glass (MSBB). Both are scored from 1 (lowest) to 5 (highest). Children (293) in grades 5 and 6 in Tallahassee, Florida (nonfluoridated) were tested for S. mutans levels. Scores of children by both tests were significantly similar (chi 2, regression and Kappa statistical analysis). Correlation coefficients (regression) between scores and DMFS increments of the previous 4 yr were 0.35 (MSBA) and 0.26 (MSBB). Both tests were very good in identifying children with low caries increments, but positive scores did not correlate well with high caries increments. Sampling and interpreting took 2 min (MSBA) and 1 min (MSBB). Cost of MSBA/child was $1.15, and MSBB/child $1.25. These tests are economical and suitable for mass screenings to identify low risk populations who do not require preventive treatment.
对两种用于定量唾液变形链球菌水平的简单微生物学检测方法进行了相互比较，并评估了其在大规模筛查和私人诊所中的适用性。两种检测方法均使用含杆菌肽的唾液链球菌培养基（MSB），且对变形链球菌具有选择性。一种检测方法评估琼脂上生长的菌落（MSBA），另一种评估肉汤中生长并附着于玻璃的菌落（MSBB）。两种方法的评分均从1（最低）到5（最高）。对佛罗里达州塔拉哈西市（未进行氟化处理）五、六年级的293名儿童进行了变形链球菌水平检测。两种检测方法对儿童的评分显著相似（卡方检验、回归分析和kappa统计分析）。评分与前4年DMFS增量之间的相关系数（回归分析），MSBA为0.35，MSBB为0.26。两种检测方法在识别龋增量较低的儿童方面都非常有效，但阳性评分与高龋增量的相关性不佳。MSBA的采样和判读耗时2分钟，MSBB耗时1分钟。MSBA检测每个儿童的成本为1.15美元，MSBB为1.25美元。这些检测方法经济实惠，适用于大规模筛查，以识别不需要预防性治疗的低风险人群。
