Tilch C, Elias P S
Mutat Res. 1984 Oct;138(1):1-8. doi: 10.1016/0165-1218(84)90078-8.
EPG and an in vitro digest of EPG by pepsin and pancreatin simulating mammalian digestion have been examined for genotoxicity in 4 mutagenicity tests employing different genetic endpoints. In the Salmonella reverse mutation assay, EPG showed only slight mutagenic activity against TA100, a strain responsive to base-pair exchange activity, in the presence of S9 mix. In vitro EPG was mutagenic for CHO-K1-BH4 cells with or without metabolic activation, the activity being greater in the presence of metabolic activation. In the in vitro SCE test, EPG was clastogenic for CHO-K1-BH4 cells independent of metabolic activation. EPG also induced transformation of C3H T10 1/2 mouse fibroblasts in vitro, producing both type II and type III foci. Subjecting an EPG solution to a simulated mammalian digestion process lowers the genotoxic activity of the solution.
已在采用不同遗传终点的4种致突变性试验中，对EPG以及通过胃蛋白酶和胰酶模拟哺乳动物消化对EPG进行体外消化后的产物进行了遗传毒性检测。在沙门氏菌回复突变试验中，在存在S9混合物的情况下，EPG仅对TA100（一种对碱基对交换活性有反应的菌株）表现出轻微的诱变活性。体外EPG对有或无代谢活化的CHO-K1-BH4细胞具有诱变性，在有代谢活化的情况下活性更高。在体外姐妹染色单体交换试验中，EPG对CHO-K1-BH4细胞具有断裂剂作用，与代谢活化无关。EPG还在体外诱导C3H T10 1/2小鼠成纤维细胞转化，产生II型和III型集落。使EPG溶液经过模拟哺乳动物消化过程会降低该溶液的遗传毒性活性。
