Ikeno K, Kuzuya H, Ishiguro I
Arch Oral Biol. 1984;29(9):669-74. doi: 10.1016/0003-9969(84)90170-5.
The changes during infancy of trypsin-like protease activity in the submandibular glands of male and female rats (4-20 weeks of age) were observed. Activity was low at 4 weeks of age, then increased markedly at 8-10 weeks of age. Seven peaks (I-VII) with isoelectric points of 4.0, 4.3, 4.5, 5.0, 5.4, 5.7 and 6.4 of protease activity were observed in the glands of adult rats by isoelectric focusing. Only peaks I and II were detected in the glands at 4 weeks of age in both sexes. After 8 weeks, all 7 peaks appeared in the glands of males and females. Repeated injection of isoprenaline into rats of 7 weeks of age elevated markedly the weight of submandibular glands; the elevation of protease activity and of inhibitory activity against dopa-decarboxylase was not found. These results indicate that proteases in peaks I and II appear at an age close to that of development of acini; protease activity in peaks III-VII increases is close to the time of development of the granular ducts.
观察了雄性和雌性大鼠（4 - 20周龄）下颌下腺中类胰蛋白酶活性在婴儿期的变化。4周龄时活性较低，然后在8 - 10周龄时显著增加。通过等电聚焦在成年大鼠的腺体中观察到蛋白酶活性的7个峰（I - VII），其等电点分别为4.0、4.3、4.5、5.0、5.4、5.7和6.4。在4周龄时，两性的腺体中仅检测到峰I和峰II。8周后，雄性和雌性的腺体中均出现了所有7个峰。对7周龄的大鼠反复注射异丙肾上腺素可显著增加下颌下腺的重量；未发现蛋白酶活性和对多巴脱羧酶抑制活性的升高。这些结果表明，峰I和峰II中的蛋白酶出现在接近腺泡发育的年龄；峰III - VII中的蛋白酶活性增加接近颗粒导管发育的时间。
