Maruyama S, Sugiura M, Nakazawa M, Tomiyama H, Shizawa M, Ishido T, Asano G
J Immunol Methods. 1984 Nov 16;74(1):173-80. doi: 10.1016/0022-1759(84)90378-8.
An indirect rosette assay for detecting islet cell surface antibodies (ICSA), the autoantibodies directed to the membranous antigens of islet cells, has been developed. In this assay, Protein A-conjugated sheep red blood cells (SRBC) were used. Both viable and fixed SRBC were available. The results obtained using this new method in insulin-dependent diabetes mellitus (IDDM) patients were not only sensitive, but also correlated with results of the indirect immunofluorescence method currently used for detecting ICSA. Furthermore, in studies of streptozotocin-treated diabetic mice or rats, the courses of ICSA appearance were successively demonstrated by this indirect rosette formation assay. Special advantages of this method are that materials are easily obtained and cost-effective, as the sample volume is small and the number of islet cells required is also small. Assays can be readily performed without a need for radioisotopic or fluorescent equipment. This method provides a reliable laboratory test for diagnosis of autoimmune diabetes mellitus.
已开发出一种用于检测胰岛细胞表面抗体（ICSA）的间接玫瑰花结试验，ICSA是针对胰岛细胞膜抗原的自身抗体。在该试验中，使用了与蛋白A偶联的绵羊红细胞（SRBC）。活的和固定的SRBC均可使用。使用这种新方法在胰岛素依赖型糖尿病（IDDM）患者中获得的结果不仅灵敏，而且与目前用于检测ICSA的间接免疫荧光法的结果相关。此外，在对链脲佐菌素处理的糖尿病小鼠或大鼠的研究中，通过这种间接玫瑰花结形成试验相继证明了ICSA出现的过程。该方法的特别优点是材料容易获得且成本效益高，因为样本量小且所需的胰岛细胞数量也少。无需放射性同位素或荧光设备即可轻松进行检测。该方法为自身免疫性糖尿病的诊断提供了可靠的实验室检测。
