Bell E, Moore H, Mitchie C, Sher S, Coon H
J Exp Zool. 1984 Nov;232(2):277-85. doi: 10.1002/jez.1402320215.
A living thyroid gland equivalent has been fabricated with a cultivated strain of rat thyroid cells (FRTL), dermal or thyroid fibroblasts, and matrix materials. The mixture becomes tissuelike in vitro by virtue of interactions between fibroblasts and collagen. Initially in vitro the thyroid cells are uniformly distributed as single cells and a small number of clusters containing less than 10 cells. When implanted into thyrodectomized hosts the thyroid cells in the tissue lattice become organized into follicles containing a colloidlike material. The follicles were found in clusters in sizes up to 0.3 mm. The clusters are vascularized. It is thought that they arise within clones rather than by an aggregation process. Using an antithyroglobulin antibody it was shown that both thyroid cells and the colloidlike material within follicles reacted positively. Development of follicles was strictly dependent on whether hosts were thyroidectomized. In nonthyroidectomized hosts no follicles were observed. We conclude that an organotypic structure can develop in vivo from a "gland-equivalent" fabricated with adult cells and matrix materials combined in vitro, and that cells cultivated for years in vitro retain the capacity to express differentiated functions in a reconstituted organ equivalent in vivo.
利用大鼠甲状腺细胞（FRTL）培养株、真皮或甲状腺成纤维细胞以及基质材料，构建了一种具有活性的甲状腺等效物。由于成纤维细胞与胶原蛋白之间的相互作用，该混合物在体外形成类似组织的结构。最初在体外，甲状腺细胞以单细胞形式均匀分布，还有少量包含少于10个细胞的细胞簇。当植入甲状腺切除的宿主中时，组织格架中的甲状腺细胞会组织形成含有类胶体物质的滤泡。发现滤泡成簇存在，大小可达0.3毫米。这些细胞簇有血管化现象。据认为它们是在克隆体内产生的，而非通过聚集过程形成。使用抗甲状腺球蛋白抗体显示，滤泡内的甲状腺细胞和类胶体物质均呈阳性反应。滤泡的发育严格取决于宿主是否进行了甲状腺切除。在未进行甲状腺切除的宿主中未观察到滤泡。我们得出结论，由体外组合的成年细胞和基质材料构建的“腺体等效物”能够在体内发育出器官型结构，并且在体外培养多年的细胞在体内重构的等效器官中仍保留表达分化功能的能力。
