Gong C M, Ho D W, Field P R, Murphy A M
J Virol Methods. 1984 Nov;9(3):209-21. doi: 10.1016/0166-0934(84)90027-2.
An indirect solid-phase enzyme-linked immunosorbent assay (ELISA) for the determination of specific IgM and IgG antibodies to echovirus type 11 in a single dilution of serum was developed using partially purified echovirus type 11 bound to microplates. Whole serum was used for IgG antibody but prior to assaying for IgM antibody interfering IgG was removed by ion exchange chromatography. The ELISA for echovirus type 11 IgG antibody was a more sensitive, rapid, technically easier and less costly alternative to the neutralisation test. With the IgG ELISA 12 of 132 sera (10.6%) known to contain enterovirus antibodies other than echovirus type 11 were positive but it could not be determined to what extent this was due to the greater sensitivity of the ELISA or cross-reactions. The IgM ELISA was even more sensitive than the IgG ELISA with acute sera, and showed a reactivity in 4 of 36 sera (11.1%) with no detectable echovirus type 11 neutralising antibodies. Echovirus type 11 IgM antibody was detected in all sera collected after the first week of infection and up to 30 days after infection. However, it was only detected in 58% of sera collected during the first week after onset thus limiting its use for rapid diagnosis. The echovirus type 11 IgM ELISA appears to have considerable laboratory diagnostic potential when a rising antibody level cannot be demonstrated in paired sera or when virus is not cultured.
利用结合在微孔板上的部分纯化11型艾柯病毒，建立了一种间接固相酶联免疫吸附测定法（ELISA），用于在血清单一稀释度下测定针对11型艾柯病毒的特异性IgM和IgG抗体。检测IgG抗体时使用全血清，但在检测IgM抗体之前，通过离子交换色谱法去除干扰的IgG。11型艾柯病毒IgG抗体的ELISA法是一种比中和试验更灵敏、快速、技术操作更简便且成本更低的替代方法。在已知含有除11型艾柯病毒之外的肠道病毒抗体的132份血清中，有12份（10.6%）通过IgG ELISA检测呈阳性，但无法确定这在多大程度上是由于ELISA的更高灵敏度还是交叉反应所致。IgM ELISA对急性期血清的检测甚至比IgG ELISA更灵敏，在36份血清中有4份（11.1%）呈反应性，而这些血清中未检测到11型艾柯病毒中和抗体。在感染第一周后及感染后长达30天采集的所有血清中均检测到11型艾柯病毒IgM抗体。然而，仅在发病后第一周采集的血清中有58%检测到该抗体，因此限制了其在快速诊断中的应用。当无法在双份血清中证明抗体水平升高或未培养出病毒时，11型艾柯病毒IgM ELISA似乎具有相当大的实验室诊断潜力。
