Orfila C, Rakotoarivony J, Durand D, Segonds-Modesto A, Suc J M
Histochemistry. 1984;81(6):533-41. doi: 10.1007/BF00489532.
The localization of beta 2-microglobulin (beta 2m) was studied in renal biopsies from 18 patients with pathological transplanted kidney using immunofluorescence and electron-immunohistochemical techniques; the renal biopsies of 4 cases with normal kidneys were used as controls. Using immunofluorescence, beta 2m was not observed in the normal kidneys, beta 2m was found in the glomeruli (7 cases) and the tubular epithelium (16 cases) of the transplanted kidneys. Using immunoelectron microscopy, some labelling of the normal kidneys was observed mainly along the cell coat of foot processes and in tubular-epithelial lysosomes. In the glomeruli of transplanted kidneys, particularly in cases of acute or chronic rejection, beta 2m was most frequently localized on the outer layer of the basement membrane and along the cell coat of foot processes. The brush border of the proximal tubules and the lysosomal structures were intensely labelled. Although immunoelectron-microscopy studies are unable to discriminate between the localization of beta 2m in normal and transplanted kidneys, these findings nevertheless suggest the glomerular filtration of beta 2m and its metabolism in the tubular epithelium.
采用免疫荧光和免疫电镜技术，对18例移植肾病理患者的肾活检组织中β2微球蛋白（β2m）的定位进行了研究；选取4例正常肾脏的肾活检组织作为对照。免疫荧光检查显示，正常肾脏中未观察到β2m，而移植肾的肾小球（7例）和肾小管上皮（16例）中发现了β2m。免疫电镜检查发现，正常肾脏中一些标记主要沿足突细胞被膜和肾小管上皮溶酶体分布。在移植肾的肾小球中，尤其是急性或慢性排斥反应的病例，β2m最常定位于基底膜外层和足突细胞被膜。近端肾小管刷状缘和溶酶体结构被强烈标记。虽然免疫电镜研究无法区分β2m在正常肾脏和移植肾脏中的定位，但这些发现仍提示β2m的肾小球滤过及其在肾小管上皮中的代谢。
