Wimpenny J W, Waters P
J Gen Microbiol. 1984 Nov;130(11):2921-6. doi: 10.1099/00221287-130-11-2921.
The Szybalski wedge plate technique was modified to generate two-dimensional diffusion gradients for two environmental variables: pH value and NaCl concentration were selected in this study. After experimentation, pH values chosen ranged from 3.9 to 8.1 and salt concentrations from 23 to 79 g 1(-1). The pH gradients were slightly sigmoidal whilst the salt gradients were approximately linear. The reproducibility of the technique was assessed and found to be satisfactory in replicate growth experiments. A wide range of growth patterns was observed for a large variety of different bacterial types. The growth boundary and the pattern details are sufficiently distinct to suggest that the technique can distinguish between closely related species or strains.
对斯齐巴尔斯基楔形平板技术进行了改进，以生成针对两个环境变量的二维扩散梯度：本研究选择了pH值和NaCl浓度。经过实验，所选的pH值范围为3.9至8.1，盐浓度范围为23至79 g·L⁻¹。pH梯度呈轻微的S形，而盐梯度近似线性。对该技术的可重复性进行了评估，发现在重复生长实验中结果令人满意。对于多种不同的细菌类型，观察到了广泛的生长模式。生长边界和模式细节足够清晰，表明该技术可以区分密切相关的物种或菌株。
