胆红素与白蛋白的共价偶联。

Covalent coupling of bilirubin to albumin.

作者信息

Jacobsen C

出版信息

Int J Pept Protein Res. 1976;8(3):295-303. doi: 10.1111/j.1399-3011.1976.tb02507.x.

DOI:10.1111/j.1399-3011.1976.tb02507.x

Abstract

A method for covalent coupling of bilirubin to albumin is described. Human serum albumin-bilirubin (1:1 complex) has been treated with water soluble carbodiimide in order to obtain covalent coupling of bilirubin to albumin. The reaction conditions have been varied with respect to pH, reaction time and concentration of reagent to obtain the optimal coupling. The prepared albumin-bilirubin compounds were investigated by spectrophotometry, gel filtration and gel electrophoresis to ascertain the covalent nature of the bond and to characterize the products further. Gel electrophoresis and gel filtration showed that a monomer fraction could be prepared, and this fraction was a suitable material for further studies.

摘要

描述了一种胆红素与白蛋白共价偶联的方法。人血清白蛋白 - 胆红素（1:1复合物）已用水溶性碳二亚胺处理，以实现胆红素与白蛋白的共价偶联。针对pH、反应时间和试剂浓度改变反应条件以获得最佳偶联效果。通过分光光度法、凝胶过滤和凝胶电泳对制备的白蛋白 - 胆红素化合物进行研究，以确定键的共价性质并进一步表征产物。凝胶电泳和凝胶过滤表明可以制备单体级分，并且该级分是用于进一步研究的合适材料。