Suzuki M, Sharma B, Waldeck N, Charles M A
Diabetes Res. 1984 Nov;1(4):179-81.
Since multiple mechanisms of antibody induced cellular killing have been described and since the mechanisms of complement augmented antibody-dependent cellular killing are poorly understood, we investigated the specificities of several monoclonal antibodies. Four murine monoclonal anti-rat pancreatic islet antibodies were evaluated for complement-dependent antibody-mediated cytotoxicity, antibody-dependent cellular cytotoxicity and complement-augmented antibody-dependent cellular cytotoxicity. Although all monoclonal antibodies were selected for islet binding properties, only 1 antibody mediated all 3 mechanisms of killing. Another monoclonal antibody which did not induce complement-dependent antibody-mediated cytotoxicity was effective in the complement-augmented antibody-dependent cellular cytotoxicity assay. These studies indicate functional multiple activities of monoclonal antibodies to islet target cells.
由于已经描述了抗体诱导细胞杀伤的多种机制，并且由于补体增强的抗体依赖性细胞杀伤机制了解甚少，我们研究了几种单克隆抗体的特异性。评估了四种鼠抗大鼠胰岛单克隆抗体的补体依赖性抗体介导的细胞毒性、抗体依赖性细胞毒性和补体增强的抗体依赖性细胞毒性。尽管所有单克隆抗体都是根据胰岛结合特性选择的，但只有1种抗体介导了所有3种杀伤机制。另一种不诱导补体依赖性抗体介导细胞毒性的单克隆抗体在补体增强的抗体依赖性细胞毒性试验中有效。这些研究表明针对胰岛靶细胞的单克隆抗体具有多种功能活性。
