对二十种野生型酵母菌株线粒体基因组ori序列的比较研究。

A comparative study of the ori sequences from the mitochondrial genomes of twenty wild-type yeast strains.

作者信息

Faugeron-Fonty G, Le Van Kim C, de Zamaroczy M, Goursot R, Bernardi G

出版信息

Gene. 1984 Dec;32(3):459-73. doi: 10.1016/0378-1119(84)90020-9.

Abstract

The ori sequences of the mitochondrial genomes of 20 wild-type strains of Saccharomyces cerevisiae were compared with those of the previously studied strain A (de Zamaroczy et al., 1984). The seven canonical ori sequences of this strain appear to be present in all strains tested, but in most strains ori1 is replaced by an extensively rearranged ori1 * sequence, and an additional ori sequence, ori8, is present between the oxi3 and the 15S RNA genes; one strain, B, lacks ori4. The location and orientation of ori sequences of three strains, B, C and K, were found to be the same as in strain A. The primary structures of four ori sequences from three different strains (ori1 of strain J69-1B, ori3 and ori5 of strain K, ori6 of strain D273-10B) were found to be identical with the corresponding ori sequences previously investigated. Hybridization experiments with different ori probes indicated a conservation of ori2-ori7 sequences in all strains tested. The primary structure of a petite genome derived from strain B and carrying ori1 * is reported and discussed.

摘要

将20株酿酒酵母野生型菌株线粒体基因组的ori序列与之前研究的菌株A（de Zamaroczy等人，1984年）的ori序列进行了比较。该菌株的七个典型ori序列似乎存在于所有测试菌株中，但在大多数菌株中，ori1被一个广泛重排的ori1序列所取代，并且在oxi3和15S RNA基因之间存在一个额外的ori序列ori8；一个菌株B缺少ori4。发现三个菌株B、C和K的ori序列的位置和方向与菌株A相同。发现来自三个不同菌株的四个ori序列（菌株J69-1B的ori1、菌株K的ori3和ori5、菌株D273-10B的ori6）的一级结构与先前研究的相应ori序列相同。用不同ori探针进行的杂交实验表明，在所有测试菌株中ori2-ori7序列具有保守性。报道并讨论了源自菌株B并携带ori1的小菌落基因组的一级结构。