Gentsch J R, Hatfield J W
Virus Res. 1984;1(5):401-14. doi: 10.1016/0168-1702(84)90026-1.
Attachment of [35S]methionine-labelled mammalian type 3 reovirus to murine L cells and human HeLa cells was studied under equilibrium conditions. Cellular attachment sites could be completely saturated with 35S-labelled reovirus, indicating that specific attachment sites for reovirus are present on the surface of these cells. We calculated that L cells possess about 86000-105000 attachment sites per cell while HeLa cells possess about 126000-147000 sites per cell for type 3 reovirus. Unlabelled reovirus was highly efficient in competing for attachment by 35S-labelled reovirus to the saturable attachment sites of both L and HeLa cells, further indicating the specificity of the interaction. We also found that unlabelled reovirus competed equally well for both binding and internalization of 35S-labelled reovirus into murine L cells, suggesting that the L cell attachment site may serve as a virus entry site. Phospholipase digestion of L cells had no effect on subsequent reovirus attachment, while treatment of L cells with moderate concentrations of bromelain (but not trypsin, proteinase K or pronase) and Vibrio cholerae neuraminidase reproducibly decreased subsequent reovirus attachment. These results and those of others (Epstein et al., 1984, Virology 133, 46-55) suggest that mammalian reoviruses attach to specific cell surface receptors on at least two species of mammalian cells to initiate the infectious cycle.
在平衡条件下研究了[35S]甲硫氨酸标记的哺乳动物3型呼肠孤病毒与小鼠L细胞和人HeLa细胞的附着情况。细胞附着位点可被35S标记的呼肠孤病毒完全饱和，这表明这些细胞表面存在呼肠孤病毒的特异性附着位点。我们计算得出，L细胞每个细胞拥有约86000 - 105000个附着位点，而HeLa细胞每个细胞对于3型呼肠孤病毒拥有约126000 - 147000个位点。未标记的呼肠孤病毒在竞争35S标记的呼肠孤病毒附着到L细胞和HeLa细胞的可饱和附着位点方面非常高效，这进一步表明了相互作用的特异性。我们还发现，未标记的呼肠孤病毒在竞争35S标记的呼肠孤病毒与小鼠L细胞的结合和内化方面同样有效，这表明L细胞附着位点可能作为病毒进入位点。对L细胞进行磷脂酶消化对随后的呼肠孤病毒附着没有影响，而用中等浓度的菠萝蛋白酶（但不是胰蛋白酶、蛋白酶K或链霉蛋白酶）和霍乱弧菌神经氨酸酶处理L细胞可重复性地降低随后的呼肠孤病毒附着。这些结果以及其他研究结果（Epstein等人，1984年，《病毒学》133卷，46 - 55页）表明，哺乳动物呼肠孤病毒附着于至少两种哺乳动物细胞表面的特异性细胞受体以启动感染周期。
