Misumi J, Bouhnik J, Alhenc-Gelas F, Corvol P, Ménard J
J Hypertens Suppl. 1983 Dec;1(2):40-2.
The effects of two different amounts of pure rat angiotensinogen were investigated in a closed circuit isolated perfused rat kidney. In response to angiotensinogen, circulating levels of angiotensin I (AI) and angiotensin II (AII) immunoreactive materials were found to increase in a time and dose-dependent manner. Vasoconstrictor and renin inhibitory effects were observed in parallel with the increase in AII. Glomerular filtration rate decreased after administration of angiotensinogen to a greater extent than renal flow and filtration fraction. The characterization by high performance liquid chromatography of peptides generated showed the liberation of AI, des-Asp1AI, AII and des-Asp1AII (AIII). These findings demonstrate that administration of angiotensinogen in an isolated perfused kidney model generates AI and that renal converting enzyme and aminopeptidases are able to convert AI to AII, AI to des-Asp1AI, and des-Asp1AI and/or AII to AIII. Changes in circulating level of angiotensinogen influence the activity of the renin-angiotensin system and, therefore, renal function.
在一个封闭回路的离体灌注大鼠肾脏中，研究了两种不同剂量的纯大鼠血管紧张素原的作用。给予血管紧张素原后，发现血管紧张素I（AI）和血管紧张素II（AII）免疫反应物质的循环水平呈时间和剂量依赖性增加。血管收缩和肾素抑制作用与AII的增加同时出现。给予血管紧张素原后，肾小球滤过率的下降幅度大于肾血流量和滤过分数。通过高效液相色谱对生成的肽进行表征，结果显示有AI、去天冬氨酸1AI、AII和去天冬氨酸1AII（AIII）的释放。这些发现表明，在离体灌注肾脏模型中给予血管紧张素原可生成AI，并且肾转化酶和氨肽酶能够将AI转化为AII、将AI转化为去天冬氨酸1AI，以及将去天冬氨酸1AI和/或AII转化为AIII。血管紧张素原循环水平的变化会影响肾素-血管紧张素系统的活性，进而影响肾功能。
