Ogihara T, Higaki J, Imai N, Kumahara Y, Murakami K, Mori K, Ogawa H
J Hypertens Suppl. 1984 Dec;2(3):S239-41.
A sensitive direct human renin radio-immunoassay has been developed for clinical use. The antigen source was human renal renin purified from Haas' preparation by pepstatin-C6-sepharose affinity chromatography, this was used to prepare a specific human renin antibody. The radio-immunoassay was performed by the double antibody technique using the delayed tracer addition method. Standard curves were obtained over the range 0.2-8.0 ng/ml. Dilution curves of human renal renin and human plasma were superimposable on the standard curve. Both active and inactive renin were detected by this method, and measurements correlated well with total renin activity after trypsin activation. Intra- and inter-assay coefficients of variance were 4.6% and 5.1%, respectively. Renin concentration was higher in patients with renovascular hypertension (1.97 +/- 0.38 ng/ml, mean +/- s.d., n = 10, P less than 0.01), but lower in primary aldosteronism (0.66 +/- 0.16 ng/ml, n = 13, P less than 0.01) compared with essential hypertension (1.38 +/- 0.34 ng/ml, n = 12). This method provides a new tool for the investigation of the renin-angiotensin system in man.
已开发出一种灵敏的直接人肾素放射免疫分析法供临床使用。抗原来源是通过胃蛋白酶抑制剂-C6-琼脂糖亲和层析从哈斯制剂中纯化得到的人肾肾素，用其制备特异性人肾素抗体。放射免疫分析采用双抗体技术和延迟示踪剂加入法进行。在0.2 - 8.0 ng/ml范围内获得标准曲线。人肾肾素和人血浆的稀释曲线与标准曲线可叠加。该方法能检测活性和非活性肾素，且测量值与胰蛋白酶激活后的总肾素活性相关性良好。批内和批间变异系数分别为4.6%和5.1%。与原发性高血压（1.38±0.34 ng/ml，n = 12）相比，肾血管性高血压患者的肾素浓度更高（1.97±0.38 ng/ml，均值±标准差，n = 10，P＜0.01），而原发性醛固酮增多症患者的肾素浓度更低（0.66±0.16 ng/ml，n = 13，P＜0.01）。该方法为研究人类肾素-血管紧张素系统提供了一种新工具。
