Shlyakhtenko L S
Institute of Molecular Genetics, Academy of Sciences of the USSR, Moscow.
J Biomol Struct Dyn. 1984 Jun;1(6):1511-23. doi: 10.1080/07391102.1984.10507534.
A procedure is developed for studying the B-A transition in DNA using gel electrophoresis. The starting point has been the idea that the junction between the A and B sections, which appear within the transition interval would increase the mobility of the DNA molecules. Indeed, the mobility of DNA in a gel is shown to increase in the middle of the B-A transition due to the formation of the largest possible number of boundaries between the B and A forms. The middle of the B-A transition in supercoiled DNA appears to be shifted against the middle of the transition in open circular (as well as linear) DNA by about 1.3% towards lower ethanol concentrations under the influence of the superhelical stress.
开发了一种利用凝胶电泳研究DNA中B-A转变的方法。出发点是这样一种想法：在转变区间内出现的A段和B段之间的连接处会增加DNA分子的迁移率。实际上，由于在B型和A型之间形成了尽可能多的边界，DNA在凝胶中的迁移率在B-A转变的中间阶段会增加。在超螺旋应力的影响下，超螺旋DNA中B-A转变的中间阶段相对于开环（以及线性）DNA转变的中间阶段向较低乙醇浓度方向偏移了约1.3%。
